Chromosome techniques a manual

 · Chromosome Techniques Book Description: This laboratory manual covers the study of chromosomes in plants, animal and human systems, dealing with the protocols and principles involved. It caters to the requirements of scientists working laboratories, presenting details of the operational mechanism for use at the chromosome level.  · Full text Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (K), or click on a .  · Description. Chromosome Techniques: Theory and Practice, Third Edition focuses on chromosome research. The book first discusses pre-treatment and hypotonic treatment. Pre-treatment for clearing the cytoplasm and softening the tissues; separation of chromosomes and clarification of constrictions; and hypotonic treatment for chromosome Book Edition: 3.

This technique produces, by cross species hybridization using differentially labeled gibbon chromosome probes, a specific banding pattern on human metaphase chromosomes. If the probes are labeled with a number of fluorochromes, usually three, this allows a colorful and reproducible banding to be observed and analyzed. The basic banding methods are complemented by techniques that stain selective chromosome regions: constitutive heterochromatin, nucleolar organizer regions, and telomere regions (C, Ag-I, and T. Earlier books on the handling of plant chromosomes have not included many of the innovations in cytological techniques for many important crops that have become available in recent years, including information on associating genes with chromosomes. The aim of this book is to compile all the plant cytogenetic techniques, previously published in earlier books, into a laboratory manual. The first.

Beginning with the Introduction, the other 11 chapters are devoted to progressive advances in laboratory protocols providing minute details about: conventional methods for handling the chromosomes, smear technique, fluorescence in situ hybridization including MC-FISH, GISH, Fiber FISH, immunodetection, flow cytometry and chromosome sorting, pollen staining, cell division, chromosomes in reproduction, and karyotype analysis. Chapter 2 Conventional Methods for Handling Plant Chromosomes. Chapter 3 Smear Technique for Plant Chromosomes. Chapter 4 Fluorescence In Situ Hybridization. Chapter 5 Flow Analysis and Sorting of Plant Chromosomes. Chapter 6 Pollen Staining. Chapter 7 Cell Division. Chapter 8 Mode of Reproduction in Plants. Chapter 9 Karyotype Analysis. Normal human metaphase spread with whole-chromosome paint probe for chromosome pair number 15 in red. (d) Cross-species colour banding (RX- FISH 1) on normal human male chromosomes arranged in standard karyotype format.